For the Kota Tinggi parliamentary seat, the fight will be between Pas candidate Onn Jaafar and BN newcomer Datuk Noor Ehsanuddin Mohd Harun Narrashid.
In the state seat of Sedili, BN candidate Rasman Ithnain will wrestle it out with PKR's first-timer, 34 year-old Datuk Abdul Kadir Sainuddin.
BN's incumbent candidate Asiah Md Ariff who will be defending her fort in Johor Lama, is set to take on her male contender from Pas, Kamaldin Tahir.
All three BN contenders as with representatives from the opposition pact arrived at about 9am at the Dewan Jubli Intan Sultan Ibrahim here for the nomination.
Noor Ehsanuddin submitted his nomination papers at 9.05am with Onn following suit at 9.15am.
Rasman submitted his documents at 9.04am with his contender Abdul Kadir at 9.12am while Asiah submitted her nomination papers at 9.05am and Kamaldin at 9.12am.
When met outside the hall after an official announcement made by the Election Commission (EC), Noor Ehsanuddin, Rasman and Asiah said that they were highly optimistic about securing Kota Tinggi for BN.
"We have always been working and the fact that I am a newcomer only serves to make me an eager fighter," Noor Ehsanuddin said.
Asiah said that she is not deterred in any way to take on her contender.
"Competitions will only serve to make one better, so let the battle begin," she said with Rasman echoing her sentiments.
"We are in this together and we will make sure we emerge champions and most importantly, work to serve the people even better," Rasman said.
The trio were greeted by the anxiously awaiting crowd outside the hall compound with cries of excitement and utter euphoria filling the atmosphere.
The crowd had arrived even before 8.30am and gathered at BN's Kota Tinggi parliamentary public service centre before marching to the hall.
Joining the crowd as a symbol of moral support was Noor Ehsanuddin's predecessor Tan Sri Syed Hamid Albar.
Syed Hamid was seen waiting till the official announcement made by the EC before congratulating Noor Ehsanuddin.